what type of metastatic model you are looking for? The mouse that has activated Kras and inactivated p53 seems to a be good model for metastatic lung cancer

(Zheng S, El-Naggar AK, Kim ES, Kurie JM, Lozano G. A genetic mouse model for

metastatic lung cancer with gender differences in survival. Oncogene. 2007 Oct

18;26(48):6896-904. Epub 2007 May 7. PubMed PMID: 17486075.)

It can be majorly classified into two methods as follows.

*Tail vein injection of human malignant cancer cells into immunocompromised nude mice and evaluate later the lung tissue, which enables you to identify the extracasation and colonization into the lung tissues

*Subcutaneous injection of malignant metastatic cancer cells (i.e. A375M, B16 melanoma cells) into immunocompromised nude mice with Matrigel and then check the several organs such as brain, lungs, bone marrow, spleen etc.

 Ｉｆ you would not like to ignore the anti-tumor immune response, I strongly recommend that you should use the autograft experiments, in which murine tumor cells such as 4T1 breast cancer cells or B16 melanoma cancer cells established from mice are injected to B6 WT mice in terms of immune system. We have previously published the work focusing on CD44 variant function in the metastasis against redox stress in the pre-metastatic niche (Nat Commun. 2012 Jun 6;3:883).

I do not understand the relationship between Mercedes' question (about in vitro setting) and both Abdullah's and Go's responses (about in vivo settings) ...

To be metastatic in vitro, a given cancer cell line should be capable of escaping the plastic dishes / flasks and invade the incubator (for example) ...

A cancer in a patient is much more complicated (at the biological level) than its sole cancerous component as more than poorly modeled in a plastic flask ...

In vivo, each cancer type has its more or less specific way(s) of metastasizing ..

The attached articles could may be of help.

Best regards

Robert

Hi,

You have plenty of models. I have experience in Breast Cancer, and easy model to track metastasis in vivo is MCF7, MDAMB231 for example.