The objective of this work is to screen culture filtrates of different bioagent fungi, then obtaining adequate quantity from filtrate to determine their bioactivity. So it is quit necessary to learn about most suitable conditions for increasing the amount of fungal filtrate.
Many thanks
You can cultivate fungi in shake flasks in submerged or solid-state fermentations. Than pass the whole liquid through a filter paper or 0.22 micra filters. Thus you will have sufficient amounts of culture filtrates to analyze and test their activity.
Thanks Nikolay Vassilev for this recommendation, I am sure it is successful step.
Jawad
For obtained sufficient fungal filtates is necessary temperature, aireation etc in optimal points whenyou selecting shake flasks in submerged fermentations.
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