Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

Is there a study of a long-term usage of muscimol and its impact upon human physical and mental health?

In my psychotherapeutic practice I have a client who decided to use really small doses of muscimol regularly. He gains it from dried fruit bodies of Amanita muscaria. Although he is quite enjoying...

14 December 2020 6,020 2 View

Covid 19 deaths- why the huge disparity between Asian countries which cannot be convincingly explained by public health measures?

Sri Lanka, Vietnam, Thailand, Cambodia and Laos for example are doing extremely well, but Indonesia, Philippines, Malaysia and India not so. It doesn't seem likely that this can be wholly due to...

27 October 2020 1,795 7 View

I see a lot of research right now that is inspired by COVID-19, even outside the medical field. Has your research focus changed due to the pandemic?

My question says it all. I am wondering if research agendas across many fields have changed due to the COVID-19 circumstances, and why.

06 July 2020 3,505 6 View

Can you help with an urgent survey about teaching during COVID-19?

Update: Data collection is closed and our manuscript has been published. Thank you to everyone who participated. You can find it at: Teacher Experiences in Converting Classes to Distance...

06 May 2020 1,701 46 View

Precipitates in BL buffer (Epitect DNA bisulfite Kit)?

18 February 2020 2,386 1 View

Background noise in q-exactive orbitrap ms ?

Dear Colleges MS users. This is my very first thread on research gate. I have a new instrument - q-exactive orbitrap and i have experienced a high background ms noise in low m/z range. After...

29 January 2020 6,927 2 View

I'm looking for a laboratory-scale hydrogen separation membrane from the air?

dear all, I'm looking for a small-scale membrane for hydrogen separation from the air (or nitrogen). I need some information about available manufacturers. There is a lot of information about the...

15 December 2019 8,333 2 View

Is there anyone who could share the protocol for NLRP3 staining in the mouse paraffin embedded tissue?

Hello, I am looking for someone who performed already some IHC/IF staining in paraffin sections in mouse tissue for NLRP3. I used ab214185 and NBP2-12446 but no success so far. Thank you in advance

30 October 2019 4,354 1 View

Abaqus python, extract displacements of surface nodes, how?

Hi, I need to extract, from existing odb, displacements of nodes whhich belong to defined element surface. I use python. Till now I'm able to list the nodes labels and coordinates: N_set =...

27 March 2019 955 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Can I measure absorbance efficiency with wavelength in a spectrophotometer?

I measure the wavelength of material in a spectrophotometer and it peaks at 275 and its absorbance is 100. I mix it with an adsorbent and it adsorbs the first material, but when I measure the...

16 February 2021 7,515 5 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

What dilution do I use for my GNP solution UV-Vis and DLS characterization?

I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...

26 January 2021 530 5 View

How to calculate DNA concentration of a sample with high concentration buffer?

I want to calculate the concentration of DNA in an unknown sample. But it's buffer has a high concentration and our nanodrop don't accept that as a blank of dsDNA. Thus I dilute the buffer with...

23 January 2021 9,440 2 View

Anyone know how to dilute strong hydrogel structure?

I am working on PVA-GMA hydrogel that is a really strong polymeric structure like rubber. Because I am in the middle of analysis, it is difficult to dissolve the gel using a common solvent. I...

20 January 2021 3,501 2 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View