What are the general antibody concentrations in flow cytometry?

Hi everybody, I am trying to block Fc receptor by human IgG for a specific experiment (it's not flow cytometry), in an adherent cell, not a suspension. I know my primary antibody working...

20 September 2020 9,119 5 View

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

29 December 2019 389 4 View

What is the correct output format of the htseq package?

Hi everyone I used the HTSEQ package to count the reads. The output of this package was as follow: Is this output format correct? Is the first column the gene id and the second column is the reads...

21 December 2019 9,326 4 View

Primer blast from NCBI?

Hello How we can do in Primer blast from NCBI that What size is made of PCR product with D-Loop Primers by using DNA samples from Cervus and Capra aegagrus?

04 October 2019 9,001 3 View

Why did my HUVECs growth rate reduced after transfection?

I did transfection using lipofectamin 2000 to get stable GFP positive cell line, but after transfection and puromycin selection my HUVEC growth rate reduced. I have not seen any colony displayed...

03 June 2019 5,109 5 View

Is system dynamics a proper tool for the simulation of Cash Based Interventions impact factors?

Can System dynamics simulate cash transfer narratives well, If we consider multiple factors such as education, health and economy?

30 January 2019 7,470 1 View

How can I remove the produced HCL during esterification reaction without adding base?

In the esterification reaction that I work on, HCl is produced.Adding base ruins the expected product since it reacts with the initial materials and forms a red byproduct. The bases that I tried...

23 January 2019 4,764 3 View

In cross frequency coupling, is it possible to taking one frequency from LFP and the other from EEG ?

28 June 2018 9,305 1 View

What databases are available for Rna-seq datasets?

I'm looking for any publicly available RNA-seq data sets containing RNA-seq data related normal brast tissues.

31 December 2017 6,029 8 View

Measurement of insulin resistance in diabetic rats?

1- How can insulin resistance be measured in diabetic rats? 2- Why is HOMA-IR not valid for rodents?

19 October 2017 3,092 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View