5/10/21

Dear Fahimeh,

Check the 2 procedures shown in the attachment. It recommends using the Folin-Ciocalteu method and measuring absorbance @ 280 nm. If you decide to use A280, keep in mind that many other substances absorb at this wavelength, so specificity could be an issue.

I hope this information helps you.

Bill Colonna [email protected]

Measuring Phenolics in the Winery | American Journal of Enology and Viticulture (ajevonline.org)

Check https://www.sciencedirect.com/science/article/pii/S1995764517304790

Also check https://www.sciencedirect.com/science/article/pii/S1658365514001046

https://www.nature.com/articles/nprot.2007.100

https://www.nature.com/articles/nprot.2007.102/briefing/signup/?origin=Nature&originReferralPoint=EmailBanner

Dear Fahimeh Nazari thank you for your interestingctechnical question. In addition to the valuable references suggested by Chinaza Godswill Awuchi and Hamid Reza Farhang please also have a look at the following potentially useful articles:

Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts

Article Optimization of Phenolic Compounds Extraction from Flax Shiv...

Optimizing Extraction Conditions of Free and Bound Phenolic Compounds from Rice By-Products and Their Antioxidant Effects

Article Optimizing Extraction Conditions of Free and Bound Phenolic ...

These two articles are freely available as public full texts on RG.

Extraction, separation, and detection methods for phenolic acids and flavonoids

Article Extraction, separation, and detection methods for phenolic a...

Optimized methodology for the extraction of free and bound phenolic acids from Chilean Cristalino corn (Zea mays L.) accession

Article Optimized methodology for the extraction of free and bound p...

(see attached pdf files)

Good luck with your research! 👍

Please, keep in mind that Folin– Ciocalteu assay does not measure the total phenolics contain. The chemistry behind this assay is pretty simple. This is the reduction of polyoxomolibdate by phenols to form the reduced Molibdates, which are blue colored. But other reducing agents, such as ascorbic acid, can also reduce Mo(VI). Thus Folin– Ciocalteu method is the redox titration.

Please look at the following below links which may help you in your analysis.

Thanks