Fahimeh Zahednezhad

4 Questions 8 Answers 0 Followers

Questions related from Fahimeh Zahednezhad

Is DAPI staining protocol different in different cell lines?

I have stained MCF7 by DAPI quite OK using 0.5 ug/ml DAPI in PBS for 10 min. However, for A549, and HT29 I could not reach any staining result even by applying higher concentrations of DAPI...

03 May 2021 344 7 View

How can I remove the produced HCL during esterification reaction without adding base?

In the esterification reaction that I work on, HCl is produced.Adding base ruins the expected product since it reacts with the initial materials and forms a red byproduct. The bases that I tried...

24 January 2019 4,889 3 View

Could you guide me about choosing the appropriate coupling reagents like EDC/DCC/TBTU/... for esertification?

In order to perform an esterification reaction, I have tried acylation using oxalyl chloride but there was a very low yield. I encountered other performed reactions using coupling reagents with...

28 May 2018 5,363 5 View

Are there any approved methods for coupling a very hydrophilic compound having a carboxylic acid part and a hydrophobic alcohol or vice versa?

Could you explain an esterification method using coupling reagents or any other method between 1. a very hydrophilic compound having a carboxylic part and 2. weak hydrophobic alcohols like...

25 September 2017 8,977 7 View