What are the general antibody concentrations in flow cytometry?

Hi everybody, I am trying to block Fc receptor by human IgG for a specific experiment (it's not flow cytometry), in an adherent cell, not a suspension. I know my primary antibody working...

20 September 2020 9,119 5 View

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

29 December 2019 389 4 View

What is the correct output format of the htseq package?

Hi everyone I used the HTSEQ package to count the reads. The output of this package was as follow: Is this output format correct? Is the first column the gene id and the second column is the reads...

21 December 2019 9,326 4 View

Primer blast from NCBI?

Hello How we can do in Primer blast from NCBI that What size is made of PCR product with D-Loop Primers by using DNA samples from Cervus and Capra aegagrus?

04 October 2019 9,001 3 View

Why did my HUVECs growth rate reduced after transfection?

I did transfection using lipofectamin 2000 to get stable GFP positive cell line, but after transfection and puromycin selection my HUVEC growth rate reduced. I have not seen any colony displayed...

03 June 2019 5,109 5 View

Is system dynamics a proper tool for the simulation of Cash Based Interventions impact factors?

Can System dynamics simulate cash transfer narratives well, If we consider multiple factors such as education, health and economy?

30 January 2019 7,470 1 View

How can I remove the produced HCL during esterification reaction without adding base?

In the esterification reaction that I work on, HCl is produced.Adding base ruins the expected product since it reacts with the initial materials and forms a red byproduct. The bases that I tried...

23 January 2019 4,764 3 View

In cross frequency coupling, is it possible to taking one frequency from LFP and the other from EEG ?

28 June 2018 9,305 1 View

What databases are available for Rna-seq datasets?

I'm looking for any publicly available RNA-seq data sets containing RNA-seq data related normal brast tissues.

31 December 2017 6,029 8 View

Measurement of insulin resistance in diabetic rats?

1- How can insulin resistance be measured in diabetic rats? 2- Why is HOMA-IR not valid for rodents?

19 October 2017 3,092 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Concentration of DNA?

Hello everyone! Is a normal value 295.8 ug/ul for cDNA? If not what does it mean? Where is the mistake? The measurement was done with nano drop. Thank you!

09 February 2021 6,202 1 View

What is the reference value of THD in BLDC MOTOR speed control ?

i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?

07 February 2021 5,892 1 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

How to know the threshold limit value of a sar image...??

i want to do thresholding by segregation of water areas from non water areas for that i am using model maker in ERDAS but i am unable to get the threshold limit value of my image.

26 January 2021 8,277 1 View

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14 January 2021 8,086 37 View

What are the most important values we should teach to our students?

In one of my articles "The need for a values-based university curriculum" https://www.universityworldnews.com/post.php?story=2019092415204357 I propose that higher education should adopt a...

14 January 2021 2,304 95 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View