Hi
I need a valid reference for the normal osmolality range of erythropoietin. Can you help me in this regard?
Can everyone help me why in low doses of a drug grow impurity, but for the same drug in higher dose we have no impurity with over time?
18 January 2024 1,108 1 View
We have a problem with Diltiazem 60 tablets. We have the Dissolution profile of Diltiazem tablet cores according to the USP monograph (Diltiazem Hydrochloride Tablets). Tablet cores passed the...
01 September 2023 8,300 1 View
we have a 20 microliter volume loop, but i want to use more volume loop because of more injection volume
31 January 2023 5,222 5 View
Can someone explain the following terms? ethanol (2X): what is alternative case it? PTFE membrane with a 0.45 micro meter pore size: what is alternative case it? polyester fiber (Carpenter Co.):...
24 December 2022 408 1 View
Do you have a reliable protocol for viewing Total protein Spirulina on sds page gel?
26 May 2022 4,042 0 View
Hello everyone I inoculated C57BL/6 mice with TRAMPC2. I saw ulceration after 32 days of inoculation. anyone knows why it happens? except of injection manner, is there another factor to make this...
22 January 2022 2,596 3 View
How to increase the turbidity of DNA samples in the LAMP method? Especially at low DNA concentrations!
09 October 2021 3,764 0 View
How can I measure total phenols after hydrolysis the extraction with NaOH? If Folin– Ciocalteu reagent work to me? Your suggestion is highly appreciated
09 May 2021 1,374 7 View
I have stained MCF7 by DAPI quite OK using 0.5 ug/ml DAPI in PBS for 10 min. However, for A549, and HT29 I could not reach any staining result even by applying higher concentrations of DAPI...
02 May 2021 375 7 View
Hi everyone. I'm going to calculate the covariate after RNAseq analysis, But I can not import the input file into IGV software. Can anyone help me?
29 April 2021 3,863 1 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...
26 June 2024 5,913 3 View
Anyone can suggest me a good reference book on cancer biology that covers drug delivery and discovery as well as basic concenpts of cancer biology?
25 June 2024 9,245 1 View
Hi Everyone, I'm reaching out for some guidance regarding conducting a comparative Ct analysis with the ABI StepOnePlus system, and I hope you can help shed some light on my dilemma. Here's the...
11 June 2024 4,044 3 View
I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...
04 June 2024 6,924 0 View
Our experimental design was a 2 (social value orientation: pro-social, pro-self) * 3 (grade: 4th, 5th, 6th) * 3 (peer comparison: fair distribution, mildly unfair, extremely unfair) repeated...
03 June 2024 8,898 0 View
I analyzed cement samples exposed and unexposed to moisture over various periods. The TGA results of these samples showed variation with each repetition. However, I consistently used calcium...
22 May 2024 1,339 3 View
Hello - planning a SYBR Green qPCR study. I have primer efficiencies, including for reference genes, from 90-105% I am planning to use efficiency correction in my calculations - do I correct for...
15 May 2024 3,370 0 View
cq values for my reference coming like 22 and cq value of my target gene coming as 23 and 24 around? can i considered this value for future calculation? or what else i can do to get better cq...
14 May 2024 6,081 6 View
Despite satisfactory qPCR curves for the positive control and calibrator, I observe a lack of signal from the VIC dye (reference gene) in my experimental samples after bisulfite conversion. I have...
13 May 2024 8,026 2 View