In mixed lipid monolayers (DPPC-Cholesterol), I observe a non monotonous behavior on the elastic modulus. It shows a decreasing trend, reaches a minimum and then it increases at high frequencies.

This minimum of G' also seems to depend on cholesterol concetration as it shifts to lower frequencies with chol increase

Could chol affect the system in such a way to cause this behavior?

I assume that it has to do with the relaxation and the time scales, and due to the structural changes that chol causes to the DPPC domains.

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