I've been using actin protein like housekeeping protein in my western-blot. When I use it with fibroblast cells from mice there were no problems, but I tried with muscle samples and I saw more than one band or nothing. I used mouse and rabbit antibodies. Is it normal see that? Could someone help me or explain to me what is happening?
Muscle have a very specific protein profile and some isoform that might cross react. Last, protein extraction in muscle is complex due to the presence of muscle fiber. If you have some problem with actin control, you could use lamin A/C. It is the protein of the nuclear lamina. Very common. This protein might help you to normalize regarding something like nucleus number.
You might have two bands, one for Lamin A and one for lamin C. You could do the addition of both and use it as control.
- Badges
- Science topic
- Similar topics
- Cell Biology
- Methods