Can I use a conversion table to convert scaled scores to percentile ranges?

Is it acceptable to use a table to convert scaled scores to percentile ranges? I am writing reports for medics and have scores from a range of different neuropsych tests. Some of the tests provide...

22 December 2019 3,022 5 View

Why is the back emf of my bldc sinusoidal ?

I am testing a BLDC motor by coupling its shaft with another motor that runs at constant speed. There are no electrical connections to the tested motor. Measuring the voltage between the phases...

10 December 2019 5,714 3 View

Best brushes for manipulating free-floating brain (rat) sections?

I'm working on mounting 40um coronal brain slices (rat) from free-floating sections. I first mounted a reference series which was then stained with tol blue. Now working on a free-floating WFA...

01 September 2019 2,703 3 View

No title

30 April 2017 893 1 View

How to improve ultrasonication efficiency?

Hello everyone. I am performing a top-down synthesis of micro and nanoparticles and one of the final steps requires ultrasonication in order to achieve the desired particle size. During my...

13 December 2014 8,923 6 View

What are the possible reasons for negative absorbance in a colorimeter?

Observed during Amylase-Starch assay at various pH.

25 July 2014 9,288 3 View

What are your thoughts on (and experiences with) open post-publication peer-review?

There have been several developments related to post-publication peer-review, commenting and/or evaluation. For example, PubMed Commons, PubPeer, Publons (the latter also offering credit for...

16 July 2014 213 9 View

Can eTPH and PAHs be included in the natural breakdown of organic material in compost soil, or are they more likely derivatives of petroleum?

Three different sources of compost were tested in order to be added to the soil at a student organic farm; the first had high levels of eTPH and pesticides (including those which we just removed),...

24 June 2014 7,036 2 View

Can anyone help me with an immunoprecipitation?

I'm trying to pulldown a FLAG tagged protein. The protein tagged is well overexpressed in my cell culture (10cm diameter dishes) (detected by wb and silverstain). I extract my protein with 2,5ml...

30 March 2014 7,785 7 View

What do you use to determine sputter film thicknesses below 1nm?

For depositing sub-nm thin films

17 December 2013 4,888 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View