Can the stickiness of body secretions increase with the increase of gluten? Was gluten found in sputum?
What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...
22 June 2024 4,567 0 View
I'm doing T7E1 experiment to check CRISPR-Cas9 transfection efficiency. Although i see my PCR products in the true site on the agarose gel, i do not see any band when i do T7E1. It is as if no...
29 November 2023 816 3 View
I made such an assumption because of the high amount of saponin in alfalfa. How true? Sincerely,
18 June 2023 7,230 1 View
This is a very technical curiosity. There is very mixed information about the ancient period, especially about the first civilizations. If this information is put in order with artificial...
30 May 2023 1,888 9 View
As known lithium chloride (LiCl) is inhibitor of GSK3 beta and which binds to GSK3 beta active site instead of Mg++. So, we found lithium perchlorate from another lab and can i see same effect of...
07 January 2021 6,257 2 View
Hi All, Our study is about miRNA function. In miRNAs biogenesis, argounate proteins provide 3p or 5p selection of miRNAs with thermodynamic stability. What is this ''Thermodynamic Stability''?...
10 May 2020 3,628 0 View
I am currently tryig to do double immunfluorescent staining in myotubes but I could not be able to see a clear negative control. As you may see in the presentation, I used two different kinds of...
18 October 2018 1,885 3 View
Hello eveyone, I am trying to establish a primary myoblast culture from zebrafish but my cells does not attach after 1st passage. I am trying to establish tissue explant culture since I do not...
01 March 2018 7,554 2 View
Hi, I need a scale assessing pre and post migration trauma among refugee (or war-torn) youth. So far, I came across with a good one in Montgomery's study which she used in 2008 study. However,...
09 October 2017 8,477 2 View
I am using 293T-BFP reporter cell. I am doing gene editing with Cas9. 293T-BFP cells have repair template plasmid and when cas9 cuts BFP site on template, BFP converts GFP by adding donor...
15 September 2017 5,775 2 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...
11 July 2024 7,062 1 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,483 0 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
What is the secret behind the success of the researcher named Dariusz Prokopowicz who has a "Research Interest Score" of 11,228 and has 2,620,101 reads in ResearchGate. How was it possible for him...
06 July 2024 2,963 0 View
Organoid with Claustro can be linked to LLM Neuroplatform - FinalSpark , finalspark.com/neuroplatform/ can be linked to chatGPT ? What to do with this ? Avatar Pilots ? Studying the case of...
28 May 2024 3,302 0 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
We are planning to collect saliva from rats for protein analysis. The issue is that the amount secreted is very low, which makes it difficult to analyze. To increase salivation, we purchased...
13 May 2024 4,630 0 View
Any pointers on how to get Cryptosporidium sporozoites to glide on non-cell substrates? We've tried oocysts from a couple of different sources (WaterBourne and the production lab at U of Arizona,...
07 May 2024 4,620 0 View
What are the medical uses of Atropine?The Role of Atropine in Modern Medicine: Indications, Admini... Atropine has several medical uses across different fields of medicine. Some of the primary...
04 May 2024 1,064 1 View