I am trying to establish a primary myoblast culture from zebrafish but my cells does not attach after 1st passage. Do you have any suggestions?

Hello eveyone, I am trying to establish a primary myoblast culture from zebrafish but my cells does not attach after 1st passage. I am trying to establish tissue explant culture since I do not...

01 March 2018 7,428 2 View

Why do i observe BFP and GFP on same reporter cell?

I am using 293T-BFP reporter cell. I am doing gene editing with Cas9. 293T-BFP cells have repair template plasmid and when cas9 cuts BFP site on template, BFP converts GFP by adding donor...

15 September 2017 5,546 2 View

What is the relationship between sleeping on the right or left side and health?

Our family and some experts told us that sleeping on the left side is not good for health. However, when I looked on the internet, I read that on the contrary, it is necessary to lie on the left...

01 January 1970 1,218 3 View

Doesn't artificial sun increase drought?

01 January 1970 8,226 4 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

Most appropriate fit of a standard curve in an enzym-linked lectin assay?

Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...

03 January 2021 1,010 6 View

No title

It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

Hi, i'm trying to synthesis Graphene oxide without (NaNO3), how ever at the end of the synthesis it just black dusty solution, any idea?

here is step from journal. 1. add 3g graphite powder in 70ml H2SO4. in ice bath and constant stirring. 2. add 9g KMnO4, after a while it become green 3. heat it to 40celcius for 30minutes, it...

22 November 2020 9,622 3 View

Why the protein is not secreted to the medium?

Dear all, Why is a protein, which is expressed in HEK293 cells, not excreted to the medium? The protein remains soluble into the cell. Which could be the reasons? Thanks for your time! Best...

04 November 2020 4,398 8 View

Antibiotics removal efficiency in WWTP and Fexcreta?

I am calculating the PEC for selected antibiotic compounds and am having problems finding the readily-available online data about the Fexcreta value for my target compounds (Since the PEC...

02 November 2020 7,675 6 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

What is Dopamine?How it affects human behaviour &directly connected to neuro-psychological disorders?

We all known Dopamine is a happy/joyous hormone secrets from brain.How it changes the over all human behaviour & responsible for the well being of humanbeing. Regards, Md Osim

02 October 2020 582 4 View

What solvents can be used to dilute the Lepidopteran defence droplets (scoli glandular secretions from lepidopteran larvae)?

I am trying to analyse the chemical profile of defensive droplets from the lepidopteran larvae (T Trypanophora semihyalina ). when i collected and tried to dilute in the hexane and acetone the...

23 September 2020 2,994 3 View

What's the secret behind deep neural networks efficiency ?

21 September 2020 1,763 4 View