I want to test if the concentration of metals in some birds were influenced by age, sex or tissue. I had to run two separate models of two-way PERMANOVA, one for age x tissue and another for sex x tissue, because I didn't know the sex for young individuals. I had significant results for the factors and its interactions.
However, the software I'm using has no post-hoc test for two-way PERMANOVA.
In that case, is it "correct" if I run a series of one-way PERMANOVA's to test the interactions pairwaise, after the two-way PERMANOVA?
Yes, you can run one-way PERMANOVAs as post-hoc tests if properly corrected for multiple comparisons. Consider pairwise PERMANOVA (if available) for a more direct approach. Check assumptions (e.g., dispersion homogeneity via PERMDISP) to ensure significant results are due to mean differences, not variance differences.
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