Someone have ever tried to differentiate human DCs from cell line that resemble mo-DCs?
I recommend that you look up the recent work of Paul Fairchild and colleagues in Oxford. They have generated DC from induced pluripotent stem cells:
Gene Ther. 2012 Oct;19(10):1035-40. doi: 10.1038/gt.2011.177. Epub 2011 Nov 10.
Cross-presentation of tumour antigens by human inducedpluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
Silk KM1, Silk JD, Ichiryu N, Davies TJ, Nolan KF, Leishman AJ, Carpenter L, Watt SM, Cerundolo V, Fairchild PJ.
In addition, please check this article by the same group...
Sachamitr P, Leishman AJ, Davies TJ, Fairchild PJ. Directed Differentiation of Human Induced Pluripotent Stem Cells into Dendritic Cells Displaying Tolerogenic Properties and Resembling the CD141(+) Subset. Front Immunol. 2018 Jan 8;8:1935. doi: 10.3389/fimmu.2017.01935. eCollection 2017. PubMed PMID: 29358940; PubMed Central PMCID: PMC5766641.
The most commonly used human monocytic cell line is Thp-1 and these can be differentiated into DCs. I am linking a review below.
Article Human dendritic cell line models for DC differentiation and ...
Hi Julie Joseph ,
Have you ever tried to use THP-1 to obtain DCs?
No, I haven't. I know someone who had used MUTZ-3 cells to generate DCs. They seemed to be stable and functionally responsive.
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