What is the opinion on the development of the methods of listing research by presenting research with our names periodically?

What is the opinion of our dear colleagues on the development of the methods of listing research by presenting research with our names periodically instead of manually inserting them?

11 December 2018 1,515 3 View

What are the best plant extracts used to protect fruits from post-harvest diseases?

What are the best plant extracts used to protect fruits from post-harvest diseases?

06 July 2018 6,661 30 View

What are the foundations for developing agricultural scientific research in developing countries?

What are the priorities for developing agricultural scientific research in developing countries?

06 July 2018 1,345 24 View

Best methods to dye inclusion bodies of plant viruses to detect and distinguish viruses by microscopy?

03 April 2018 6,067 2 View

Who can identify these insect eggs on capparis plants?

04 May 2017 6,474 4 View

Is there relation between colchicine ( for FMF long term) and lung cancer?

01 February 2017 6,745 7 View

Does Xylella fastidiosa transmitted by Edwardsiana rosae?

06 July 2016 1,932 4 View

Which hypothesis proved that plant meristem tip free of viruses?

Several assumptions.

04 May 2016 6,728 1 View

How could I differentiate between Adults of Uresiphita reversalis ,U. glivata and u. polygonalis?

04 May 2016 1,574 3 View

Does Cotesia spp. Registerd as parasite on Uresiphita gilvata?

Uresiphyta spp. as pest of several plants, the main parasite to control this pest may be a solution to stop using insectisides.

04 May 2016 9,624 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View