Could anyone suggest me a protocol for rice root protoplast isolation?

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Hayat Essalmani

Rice protoplast and cell culture

April 2008

DOI10.1142/9789812814265_0068

J. A. THOMPSONEdward CockingEdward Cocking

A rapid and efficient method for isolation and transformation of rice protoplast

January 2014Zhiwu Shengli Xuebao/Plant Physiology Journal 50(3):351-357

L. DuanJ. QianX.-Y. GuoY. Zhu

you adapt these techniques to the roots (take young roots, their wall is not resistant to enzymatic digestion)

J. C. Tarafdar

Dear Diego, please have a look of the attached files.

Diego Piacentini

Dear Tarafdar,

Thanks for the files but, unfortunately, none of them talk about protoplast isolation from roots.

Please have a look of the attached files.

Taras Pasternak

Corn root protoplasts: isolation and general characterization of ion transport

W Lin - Plant physiology, 1980 - Am Soc Plant BiolA method was developed for the large scale and rapid isolation of intact viable corn root protoplasts. Pure and metabolically active protoplasts were collected using a flotation technique. Vital staining tests, light and electron microscopy, and measurements of basic … Cited by 82 Related articles

This is not a root protoplast, this is a callus protoplats,

Mathur_Plant_Sci_Rep_14.pdf4.34 MB

Protoplast isolation and regeneration of fertile plants from arabidopsis Trp mutant trp1 100.pdf3.33 MB
What is the aim of root protoplaszts, because it is not a very good system because of high heterogenity (in rice it is ven more visible). ,