Could anyone suggest me a gene involved in Chromosomal abbrevation in onion root tips and its primer sequence?

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How to obtain slope distribution of an AFM scan using Nanoscope?

I have AFM scans of a uniformly textured surface of 16x16um size. I would like to obtain the slope distribution of the surface using Nanoscope, as I plan on trying to relate this parameter to the...

10 April 2024 5,024 2 View

Suggest any good article on Gel Propellants used in aerospace applications. Also are gel propellants currently in use?

gel propellant, rheology, aerospace

23 January 2024 9,151 1 View

Can anyone suggest a free citation manager alternative to Mendeley?

Over the last 10 years, I have been using the Mendeley citation manager. Due to their new policy, it is not working as smoothly with MS Word. Therefore, I am looking for a good alternative.

10 January 2024 3,460 5 View

Is the guide RNA (gRNA) designed for gene knockout has possibility of binding to messenger RNA (mRNA) of the target gene?

In CRISPR/Cas, we design guide to make double strand break in a specific site of the genomic DNA. I am curious to know, whether the gRNA-Cas9 complex has any possibility to bind to the messenger...

25 December 2023 630 1 View

If I want to knock out an essential gene from E.coli genome and replace it with a homologous gene from another genus. What should be the strategy?

If I want to knock out an essential gene from E.coli genome and replace it with a homologous gene from another genus. What should be the strategy I Should follow

25 October 2023 3,773 2 View

How to define predefined field in Friction Stir welding simulation using CEL Method?

I am trying to simulate FSW in CEL using abaqus. I am trying to replicate exactly the same simulation of this paper :Coupled Eulerian Lagrangian finite element modeling of frict... . But the...

04 August 2023 3,782 0 View

How to simulate evaporation through porous media with evaporation-condensation method in ansys fluent?

in the model I am simulating, the mixture of ethylene glycol and water is flowing in the hollow fiber membrane (a cylindrical hollow tube made up of porous media). during the flow, the water in...

19 July 2023 7,116 1 View

How to couple thermal boundary conditions such as covection in SPH method in Ansys LS-Dyna?

I have been trying to simulate the Friction Stir Welding process in Ansys LS-Dyna using the SPH method. I am struggling to couple it thermally to find the temperature, add convection, etc. Please...

12 July 2023 8,181 0 View

How to resolve this error '***ERROR: Excessive rotation of node number TOOL-1.81743' ?

I am trying to simulate Friction Stir welding using the CEL method. I have resolved all other errors except this particular error. Someone kindly help me with this. I have attached the CAE file.

11 July 2023 8,959 2 View

Sanger sequencing giving multiple peaks after few base pairs. What are the possible reasons? How can I confirm that? Suggest me way to get the seq ?

I am analyzing the gene sequence of my target gene in Brassica juncea. In that there is some ambiguities in the gene sequence which is appearing after few clear peaks in the sequence. I am...

27 June 2023 9,927 1 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View