I've been searching for researches on above topic, but still needs some practical methods and applications, could anyone with such background help me? Thanks!
Could you be more specific about what you mean by "discrimination" and whether there is any particular type or function of membrane transport proteins you are interested in?
Thanks for your answer! By "discrimination", I want to first identify which chemical does this membrane transport protein transport, then figure out whether it's involved in active or passive transportation, and determine which energy driving force does it employ if it's involved in active transportation.
Besides, I'm especially interested in ion channels and their function in chemesthesis, like their interaction with phytochemicals like capsaicin, menthol and mustard oil. Thanks again!
Two frequently used electrophysiology methods for studying ion channel function are (1) patch clamping of membranes in which the protein is overexpressed and (2) single channel conductance measurements of black lipid membranes reconstituted with the purified protein. These systems allow experimental manipulation of the ions in the system, establishment of transmembrane potentials, and addition of pharmacological agents.
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