How do you Evaluate Telomere length through qPCR?

Hello Everyone, I am Venkat and am trying to work with cells and tissues from the aorta. I am hoping to study telomere lengths from my samples and I was wondering if there were a reliable way to...

07 December 2017 8,250 7 View

What buffer can replace the binding buffer in DNA purification kits?

I have a QIAquick spin column but do not have the PB and PE buffers provided with it. I do know however that the binding buffer needs to have a chaotropic salt. Is there any buffer/solution with...

04 June 2017 1,750 2 View

Are you familiar with Cp values for 18S rRNA?

14 April 2015 7,664 28 View

Why or how does serum reduce the transfection efficiency?

24 March 2015 9,996 6 View

Why do most vectors have 2 origins?

I am using two different systems for my RNAi screen and both of them seem to have 2 origins of replication and I am not clear what the reason is? One of them has a f1 and pUC ori and the other has...

06 March 2015 2,685 6 View

Is there any particular reason for using HEK cells for lentiviral packaging?

Hi all, dor lentiviral packaging, I used both HeLa and HEK cells and I found out that packaging had occurred in HEK but not in HeLa. Is there any particular reason for that? Has anyone tried...

20 November 2014 5,279 7 View

How to get the Standard Deviation?

I had used Permutation in Plink to check the validity of my markers after 10000 permutations. I used the command --mperm 10000 for permutation. Is there a way to get the Standard Deviation of the...

06 March 2013 1,963 7 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View