Please suggest me the solvent to be used to dissolve Forskolin as it is insoluble in water. We have tried to solubilise it in 10% DMSO. But after adding PBS or water, the solution turns into the precipitate. I wanted to inject Forskolin directly into the brain nuclei so accordingly kindly suggest the maximum percentage of DMSO to be used.
Thanks in advance.
First you could do a ternary diagram using mixes of water/DMSO at different proportions to investigate the minimum amount of DMSO needed to solubilise your Forskolin. Also you could add surfactants such as lecythin or phosphatidin choline into the PBS before adding the DMSO solution of forskolin with strong stirring to obtain an emulsion, Third: you could use beta-hydroxycylodextrine dissolved in water or PBS to form an inclusion complex 1:1 and perhaps obtain a solution. These are only a few ideas that you may consider.
I suggest to prepare stock solutions of 10mM forskolin in 100% DMSO, which is a standard stock solution we have been using in the lab for years. What is the amount that you want to inject?
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