Could anyone please suggest
1) a good cytokeratin antibody for immunofluorescence microscopy
2) a fixation protocol for cytokeratin in HEK 293 cells?
Can anyone please share any article showing the minimum volume of sample/ protein concentration to perform a Laser ionization mass spectrometry (LIMS) or any mass spectrometry techniques in general?
12 August 2022 3,361 4 View
Can anyone please suggest a cheap hydrogel brand available to buy for 3D cell culture? Or if someone can share a recipe to prepare homemade hydrogel (for 3D cell culture), that would be fantastic.
13 May 2022 8,369 4 View
I tried to grow monolayer of cells (I use HEK 293) on agarose and acrylamide gels, I am not able to get the monolayer. Can anyone please share a working protocol?
08 April 2022 885 0 View
All Silicones are soluble in water or insoluble!
01 January 1970 3,556 6 View
Are the mother and daughter centrioles perpendicular to each other in all types of cilia basal bodies/ MTOC?
01 January 1970 289 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View