Jarshad Jas

7 Questions 2 Answers 0 Followers

Questions related from Jarshad Jas

Could anyone please suggest a good cytokeratin antibody and a fixation protocol for HEK 293 cells?

Could anyone please suggest 1) a good cytokeratin antibody for immunofluorescence microscopy 2) a fixation protocol for cytokeratin in HEK 293 cells?

04 October 2024 6,513 0 View

Does anyone know what these black dots on HEK 293 cells are?

Does anyone know what these black dots on HEK 293 cells are? They are dividing nicely and seem healthy. Is this normal?

15 August 2024 6,901 3 View

Are there any COS-7 cell lines available for purchase that constitutively express any fluorophore-tagged cytoskeleton proteins ?

Are there any COS-7 cell lines available for purchase that constitutively express any fluorophore-tagged cytoskeleton proteins (such as actin or microtubules)?

04 March 2024 9,575 0 View

Minimum volume of sample to perform a mass spectrometry?

Can anyone please share any article showing the minimum volume of sample/ protein concentration to perform a Laser ionization mass spectrometry (LIMS) or any mass spectrometry techniques in general?

13 August 2022 3,373 4 View

Can anyone please suggest a cheap hydrogel brand available to buy for 3D cell culture?

Can anyone please suggest a cheap hydrogel brand available to buy for 3D cell culture? Or if someone can share a recipe to prepare homemade hydrogel (for 3D cell culture), that would be fantastic.

14 May 2022 8,384 4 View

Does anyone know how to get a monolayer of cells (HEK293) on a hydrogel?

I tried to grow monolayer of cells (I use HEK 293) on agarose and acrylamide gels, I am not able to get the monolayer. Can anyone please share a working protocol?

09 April 2022 897 0 View

Are the mother and daughter centrioles perpendicular to each other in all types of cilia basal bodies/ MTOC?

01 January 1970 301 1 View