Could anyone help me by providing a easy and robust sample preparation protocol to quantify drugs in cell culture media and lysate for LC-MS analysis?

More Md. Mamunur Rashid's questions See All

Which would be the best colon cancer cell line to induce cancer in mice? and which mice model would be the best choice to induce colon cancer?

08 September 2017 8,948 11 View

How can I quantify a list of unknown metabolites without having its standard?

Hi, I found some new metabolites of a compound through urine sample analysis and I wanted to quantify those metabolites using UPLC-MS/MS. The metabolites standard is not available. Therefore,...

05 June 2016 1,168 13 View

What are the advantages of using linear regression model over the quadratic regression while checking linearity in a quantitative analysis?

Why most of the reviewers prefer linear regression model for making calibration curve? Need your valuable opinions....

01 February 2016 2,393 6 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

No title

02 March 2021 3,060 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

No title

01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Elena V Romanova

I suggest that you contacted Phenomenex. They will be happy to recommend you (for free) their protocols and products that suit your class of drugs best. Good Luck!

https://www.phenomenex.com

Md. Mamunur Rashid

Thanks to both of you for your assistance. Have a nice time.

Navid J. Ayon

Sample preparation will depend on the type of drug and cell matrix. Organic solvents, acid treatment, sonication etc can lyse the cells and get intracellular content for quantifying the drugs being taken inside the cell. Before you do that you have to check the matrix effect. You would require to remove proteins and other unwanted contents to reduce matrix effect for which you would require wither solid phase extraction, liquid-liquid extraction, syringe filtration etc. For this type of study an internal standard greatly help to calculate the recovery from matrix and also in data normalization. But you have to be more specific to get a better answer. In short, there is not a universal protocol for this which will work for all analytes in all cell lines/biological samples.