Could any one please let me know which transfection method is best to under express gene in Hacat cells?

17 February 2022 1 9K Report

I Tried doing with lipofectamine RNAiMAX but I could not see much fold change.

hi Ananya Sp

You can try polyplus transfection reagents. In addition, Serum starvation enhances transfection efficiency. Keep any positive control.

Cheers!

Badges
Science method

More Ananya Sp's questions See All

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning climate change?

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning time-dependent temperature, relative humidity and concentration of carbon dioxide?

08 February 2021 4,277 1 View

How to reduce the time taken for simulations in NetSim?

When I run large simulations with 100s of devices it takes several hours for the simulation to complete. So if I need to run like 20 - 30 different parameter settings then it would take me several...

05 November 2020 7,812 2 View

How can I automatically run a large number of simulation in NetSim?

I want to do a multi-parameter sweep by changing the value of one parameter for each run. It is cumbersome to do it via GUI and I wanted to know how to automate this in NetSim. Thank you.

13 October 2020 5,283 3 View

Does anybody have full research paper of British Journal of Educational Technology?

Due to my research purpose, I want a full paper of British Journal of Educational Technology, does anybody availed here its link?? On its site, there is only abstract or charging high price, please

09 April 2020 3,715 3 View

How to learn to make a strong research methodology for my work?

What does the meaning and way to make a strong research methodology. I requested many researcher in education field personally but none of them took interest. I want to grow more in research.

09 April 2020 5,324 4 View

In case of a symmetric full cell do the current collectors differ?

I am making a symmetric sodium ion full cell using NASICON type materials, i.e anode and cathode materials are same. So, do I need to use aluminium and copper as current collectors for cathode and...

22 February 2020 1,287 2 View

Is there a Non destructive method to test bulk Gold jewelry for its purity?

Currently, XRF (karatmeter) used are only surface level measurements. And for bulk purity analysis of the product it has to be melted and tested using Fire Assay process. Is there a technology or...

12 December 2019 828 3 View

Why does the charge transfer resistance of some materials decrease after cycling?

I am using WS2 as anode material for Li ion and Na ion batteries. EIS data shows that the diameter of the circle is decreasing with increasing number of cycles, indicating charge transfer...

16 November 2019 6,899 3 View

Why there are only 14 Bravais lattice types?

There are 7 Bravais lattice systems. Ideally each system should have four types, namely, primitive, base centred, body centred and face centred. However, only orthorhombic has all 4 types and...

16 September 2019 2,140 1 View

What is shuttle effect in Na-S batteries?

How does the formation of polysulfides reduce the capacity of the system?

16 May 2019 2,634 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

Why the cells die after stable transfection?

I tried stable transfection in the U87 cell line with the sgRNA-Cas9 All-in-One lentivector. Cells were cultured in a 6-well plate, 4ug plasmid was transfected after 24hs when the cell confluence...

22 February 2021 2,864 4 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View