HPLC question: I have a solution with an unknown concentration but I also don't have the response factor, is it possible to calculate the concentration? Even if it won't be accurate.
No! You need a curve of detector response to mass (concentration) in order to plot your unknown (since y = mx + b for a linear curve).
Bruce Neagle is right, quantification is not possible without a response factor. If you know the concentration of any other component in your chromatogram you may be able to estimate the concentration of your analyte, but this is very inaccurate as it does not take into account response differences between analyte and reference peak. The error can therefore be very large. I would not recommend this and would at best use it as information about the order of magnitude of the concentration.
Best regards
Joachim Horst
If you have a solution with an unknown concentration and you don't have the response factor, it is not possible to accurately calculate the concentration using HPLC. The response factor is essential for relating the concentration of a compound to the detector response in HPLC. Without this factor, the calculated concentration would not be accurate. While there are some discussions and calculations available on platforms like ResearchGate and Chromforum, they all emphasize the need for a standard value or response factor to quantitatively calculate the concentration. Therefore, it is advisable to obtain the necessary standard value or response factor to ensure the accuracy of the concentration determination using HPLC.
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