Could any one kindly teach how to derive non linear curve regression for the determination of IC50 values with any of the free available software? How to incorporate multiple data in the same graph?
If you have Excel, have you explored using it? I know that I have done exactly that myself, but I can't remember whether it required an "add in" statistical analysis pack to do the nonlinear modeling (and even it does, there are a number of those available online for free on the web).
Hi, SPSS can carry out non linear curve regression with the name "curve estimation" here is link for a hopefully useful video for doing these analyses:
Have you tried to utilization of softwware Web Enabled Statistical Software for Analysis, This is freely available at www.wessa.net and it is also FREELY available
Try it. You really enjoy using its prediction and representation power
You can do it using Excel without any special/external add-in. You just need the Solver Add-in that comes with excel.
If you're willing to send and example set of data I'll share an excel workbook to perform the curve fitting (non-linear regression) as well as the corresponding chart.
Often the nonlinear correlation to be fitted to data can be 'somehow' linearized, as a first stage. Linearized correlation and the corresponding linearized plot are often quite convenient to qualitatively evidentiate scattering around the trendline and to emphasize major effects and the physical meaning of the correlation parameters. Estimates of the parameters derived by least squares after the linearized correlation (modified from a former nonlinear correlation) can possibly be refined by iterative nonlinear least-squares regression; to find unbiased least-squares estimates for the original correlation. It may be advisable to compare both the linearized and the nonlinear correlations.
The following RG discussions seemingly can be found of some interest, concerning to your query:
Carlos Araújo Queiroz It has been about 6 years since Prashanth Gopala Krishna asked the question and thanked respondents for their answers. I think he kas moved on.