Dear Experts...
Your Suggestions are needed to make Plant Tissue Culture (MS) medium.
Could Ammonium Sulphate [(NH4)2SO4] be a substitute for Ammonium Nitrate (NH4NO3) in MS Medium?
If so..! what could be the amount per liter?
Dear Badhepuri Mahesh Kumar yes, and no, it depends of many factors. However, at first, the substitution of salt composition in culture medium, force to recalculate either molar concentration of whole formulation and the replacement of an ion by other. Since, for the preparing of original MS a great quantity of NH4NO3 is added, you would have an excess of sulphates and, at the same time, a great reducion of nitrates. How this can affect the behavior of your culture, it's not known; however, it's not the MS medium anymore.
Yes Sir Ricardo Julian Licea-Moreno
Even I am thinking the same doubt here about how the excess amount of sulphate will impact plant growth...!
and you absolutely correct if we modify it, it won't be MS medium anymore.
Few reports and experts are suggesting Ammonium Sulphate has significant response on woody plant growth. we have to test our own and let confirm the impact...
Thank you for valuable comments
Dear Badhepuri Mahesh Kumar I've performed experiments in which an excess of FeSO4 (2x) of used in DKW-C caused damages to in vitro materials: stunted microshoots, small basal calli, deep yellowing of leaves... It's highly probable that increasing several times the concentrattions of SO4 ions of MS formulation, it would expected to cause great disorders on the in vitro materials
Relating your problem, first you must know what is the reason to change Ammonium nitrate by Ammonium sulphate. There is not sense, because M&S solution had been established through research on tobacco (Doctoral thesis of Professor Toshio Murashige) and is used in so many laboratories around the world.
BUT, if you want to add the Ammonium sulphate go ahead, so you have to be forced to recalculate either molar concentration of whole formulation and replace an ion by other. Wll be an additonal work for you.
Perea Dallos Margarita We want to replace it just because of the unavailability of Ammonium Nitrate in India, it is banned here
Well, if you need to change Ammoium nitrate by Ammonium sulphate, you have to recalculate the molar concentration of the solution. I hope you will have very good results.
I hope in your Institute, laboratory or University you will find people who will help to you for recalculate you solution. Any professor of chemistry or agriculture Engeneer will help to you
On predicting the pH of a pure (NH4)2SO4 aq. sol.; see: https://www.researchgate.net/post/What-is-the-difference-between-using-ammonium-sulfate-and-using-sodium-sulphate-in-protein-ppt-and-seperation
The pH of pure ammonium nitrate (NH4NO3) aq. sol. can be predicted as that for pure ammonium chloride (NH4Cl) aq. sol.; hence as I have shown at:
https://www.researchgate.net/post/What-exactly-does-adjusting-8mM-Ammonium-Chloride-to-solutions-of-pH-of-9-and-10-mean/1
Some questions sent by you with the same subject were answered in different dates. So I am in the same posistion as before.
Good look
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