Is there any method for tuning Subspace Predictive Controllers (SPC)?

My current research is about SPC and I'm interested in tuning strategies for these types of predictive control approaches. The cost function is on the photo and the tuning parameters are Np...

23 July 2020 1,176 1 View

How I can to obtain the Z-Transform of a vector of data?

I have a vector corresponding to an impulse response g (k), but I don't know to get the Z-transform G (z) of this vector. I tried to do a function in Matlab that get G (z) of definition using a...

03 October 2019 5,582 1 View

How can I remove PEI coating?

12 September 2017 2,558 6 View

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We believe that cross-fertilizing the tourism and seaport literatures on the specific topic of relationship networks for competitiveness can favour the spreading of practices and can create a...

01 December 2015 5,484 3 View

How can I statistically compare the sensitivity of several models?

04 February 2015 8,067 2 View

An open source high level package for GPR processing and visualization?

What is the best Matlab (or Python) - open source high level - package for GPR processing and visualization - to work on as an user, or to software development?What about OpenDtect/Madagascar -...

08 September 2014 6,813 13 View

Any literature suggestions on economics of science and/or technological parks?

I'm looking for papers and books about the economics of science and technological parks, theoretically and empirically. Theoretically, I want to understand the underlying logic and when science...

14 April 2014 7,160 8 View

Does anyone have suggestions on how to decrease the background of a goat IgM ELISA?

Indirect ELISA. To detect IgM antibodies against a specific pathogen, we use coated plates from a commercial kit (validated in case of IgG detection) and we replace the conjugate with an anti-goat...

17 October 2012 2,799 14 View

Collaborate in a Life Cycle Assessment Study

I am looking for anyone who knows Aspen plus and has interest in to collaborate in a life cycle assessment study about ionic liquids.

01 January 1970 597 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View