What is the difference between spark plasma sintering and electric resistance sintering?

It is well known that plasma sintering is based on the electrical spark discharge phenomenon for preparing high-density composites at lower temperatures and higher sintering speed. How it differs...

03 March 2020 4,458 4 View

Management and social science research question?

In management and social science research, what is the difference between the following- 1. A has a positive effect on B 2. A positively influences B 3. A has a positive impact on B

13 October 2017 4,332 3 View

What are the most important flavor compounds in Coffee?

I have read numerous papers regarding the chemical compounds that are most important for coffee flavor but all mention different chemical compounds with very little overlap. Obviously different...

12 September 2017 6,120 5 View

How long do NCI-H1299 cells normally take in order to adhere to a plastic cell culture surface following thawing?

How long do NCI-H1299 cells normally take to adhere to a plastic cell culture surface following thawing? and how can we improve the speed of adherence? We received the cell line from the ATCC. We...

05 April 2016 1,417 3 View

Any suggestions for plasmid/vector with both luciferase or GFP expressing and also HRE for expression of protein under hypoxic conditions?

Any suggestions for plasmid/vector with both luciferase or GFP expressing and also HRE for expression of protein under hypoxic condition. It is to construct bacteria that bioluminescence and also...

16 December 2014 7,301 1 View

How can I perform the detection and the protection of 3 phase induction motor against faults using MATLAB?

I just want to simulate the faults and the simulation of protection against such faults which occur on 3 phase induction motor using MATLAB.

29 February 2012 392 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View