My compounds is choloro benzaldehyde and it is insoluble in water , i am willing to do biotransformation in LB (Lysogeny Broth )media , what should i do?
Dimethyl formamide (DMF) is a good solvent to dissolve most organic compounds. I suggest you make a stock solution of 100 mg/mL in DMF then add 20 to 50 uL (i.e. 2-5 mg) into the 50 mL grown microbial culture for its biotransformation. Try this hope you will get it biotransformed.