What is the difference between spark plasma sintering and electric resistance sintering?

It is well known that plasma sintering is based on the electrical spark discharge phenomenon for preparing high-density composites at lower temperatures and higher sintering speed. How it differs...

03 March 2020 4,458 4 View

Management and social science research question?

In management and social science research, what is the difference between the following- 1. A has a positive effect on B 2. A positively influences B 3. A has a positive impact on B

13 October 2017 4,332 3 View

What are the most important flavor compounds in Coffee?

I have read numerous papers regarding the chemical compounds that are most important for coffee flavor but all mention different chemical compounds with very little overlap. Obviously different...

12 September 2017 6,120 5 View

CopGFP Control Plasmid amplification

14 July 2017 3,934 1 View

How long do NCI-H1299 cells normally take in order to adhere to a plastic cell culture surface following thawing?

How long do NCI-H1299 cells normally take to adhere to a plastic cell culture surface following thawing? and how can we improve the speed of adherence? We received the cell line from the ATCC. We...

05 April 2016 1,417 3 View

Any suggestions for plasmid/vector with both luciferase or GFP expressing and also HRE for expression of protein under hypoxic conditions?

Any suggestions for plasmid/vector with both luciferase or GFP expressing and also HRE for expression of protein under hypoxic condition. It is to construct bacteria that bioluminescence and also...

16 December 2014 7,301 1 View

How can I perform the detection and the protection of 3 phase induction motor against faults using MATLAB?

I just want to simulate the faults and the simulation of protection against such faults which occur on 3 phase induction motor using MATLAB.

29 February 2012 392 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

Safety of the SARS-Cov-2 mRNA vaccine?

Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...

04 February 2021 721 6 View

Can a mRNA be degraded but still observe a protein over-expression?

Hello there, I'm currently trying to over-express a gene by transfecting the cells with a pcDNA6 plasmid containing my target gene. When I measure the mRNA for my gene of interest, the levels are...

03 February 2021 4,112 4 View

Choice of plasmid for mRNA vaccine?

Which plasmids will be the best for making mRNA vaccine? What are the points for consideration when choosing suitable plasmid for such purposes?

02 February 2021 9,092 1 View