Hi I have a problem to get the mRNA from larva of hookworm. Can anyone suggest me with the procedure?
What have you tried so far and what was the result?
Hello. I am new to cell culture work. May I know whether these small black dots are contaminants or just cell debris? It's quite confusing because they are not moving. This is different from the...
02 May 2024 8,479 1 View
I did my lab and found out one patient got PI2 and PI4 positives due to fever. Hence, I need confirmation if anyone gets the same result as me.
24 April 2024 1,322 1 View
I was trying to calculate the polarization value of FE-ZB' Ga2O3 monolayer using VASP. I also tried with LCALCPOL tag in VASP, but it shows it is not an insulator, hence it will not be calculated....
24 April 2024 3,503 0 View
for example: I am using a refrigerator for 3 years , recenly, 2/3 months ago the noise level seem increased. I made a complain to the refrigertor manufacturing brand. Now, how the R&D team of...
27 February 2024 6,870 2 View
PEH can use in micro and macro system. Is Micro system feasible for PEH for application like medical implant, Wireless Sensor Network or else?
02 February 2024 1,065 2 View
Hello ResearchGate community, I'm looking to calculate the Standardized Precipitation Index (SPI) using distributions other than gamma in R. Any guidance or code examples would be appreciated ....
05 September 2023 7,540 2 View
Several studies diluted the digested solution of e-waste but what is the purpose of this procedure?
31 January 2023 5,056 1 View
My compounds is choloro benzaldehyde and it is insoluble in water , i am willing to do biotransformation in LB (Lysogeny Broth )media , what should i do?
22 August 2022 5,006 2 View
My compounds is choloro benzaldehyde and it is insoluble in water , i am willing to do biotransformation in LB (Lysogeny Broth )media , what technique might be useful which doesn't affect yeast...
22 August 2022 702 1 View
I digested (105 degree C) Polyhydroxybutyrate (PHB) polymer in Chloroform:methanol solution, and after cooling, 1 volume of the water was added, the pH of the mixture was very low (
09 March 2022 9,473 0 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
18 July 2024 10,010 0 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
11 July 2024 4,812 5 View
I am looking for a methodology to evaluate the quality of wastewater using zebrafish larvae and embryos. Test No. 203: Fish, Acute Toxicity Test generally describes the procedure, but I have...
16 June 2024 6,473 0 View
According to standard guidelines, experts suggest proper feeding ratios to grow the ZF healthly.
15 June 2024 4,354 1 View
Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?
13 June 2024 389 0 View