According to UV–vis results, the synthesized Cu NPs is ok , but samples were analysis by XRD could not determine  Cu NPs .suspension of bacteria was added to 5mM cuso4 solution  with vigorous shaking until gradually changing the color of the mixture during 15 min to dark indicating the formation of Cu NPs (as monitored by UV–vis  of the solution).

The suspension was centrifuged and dried and analysis by XRD.

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