Control for dpph assay?

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Gaurav Kumar

Hi Sriharsha,

For DPPH assay methanol is used as blank where as DPPH + methanol is used as experimental control.

Ashutosh Meher

Absorbance of DPPH + methanol is used as negative control,

A-B

% of inhibition= -------------------X 100

here A is the negative control, B is the absorbance of test or standard