Spleen cells are fused with myelomas and therefore Hybridomas are immortal? although they exhibit ploidy they somehow (~1%) manage to survive. Eventually they reach senescence and perish after 30-40 cell divisions? hayflick limit is only applicable to regular cells I suppose. So hybridomas are immortal how do we monitor how many times a single cell has divided before it dies.
Any labeling methods that are employed for this purpose?
Divaker Choubey
Cells do not die, instead, cells become senescent (if cells are not immortal). One can measure telomerase activity as well as telomere length to assess the number of cell proliferations in the primary cells (mortal cells).
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