I had these fibroblast cells in culture for one day. There is 3x Pen/Strep, Fungin and Primocin in the media. While focusing on the viewing plane above the cells I noticed a shadow that moved almost like a sheet when rocking the culture vessel. I removed the media and took the attached picture. Is this some type of yeast or fungal contaminant? I had to rinse the cells with PBS to get rid of it. Media was fresh and sterile filtered.
Hi
Once the contamination was noticed in cell culture, It should undergone for decontamination process even all the culture materials including the aliquioted media cell culture buffers other wise the slow growing fungal/mycoplasma will increase in entire cell culture facility, once decontamination was done immediately the entire facility should treat fumigation with Pottasium permanganate and formaldehyde tow times in a week , after that exposed the facility with Sterility SBCD agar media if nil colonies , then proceed with new cell line and fresh media, Best of Luck
Thanks all for the recommendations and responses! I was able to rule out fungal contamination by testing the same cell lines with different antibiotics. It looks like it is protein precipitate since I was too aggressive with the antibiotic combinations (fungin, primocin and Pen/Strep). I just added 2x anti/anti this time and cells look great!