Hello.
I am going to perform a longitudinal study in mice involving a radiotracer that is eluted in Ethanol in its formulation, [11C] Raclopride. The amount of Ethanol in the final product is no higher than 10%. I am planning to inject this very solution in the animal via i.v. and flush with a saline solution, but I am concerned with the final concentration of Ethanol in the organism being high enough to hinder the animal, or giving me some long-term effect on dopaminergic neurons that are undesirable in a longitudinal study.
Is there anyone that looked into this specific matter, or that can give me some references on it?
Thank you very much.
Hello Bruno,
I would also like to know the answer to this question! I believe Aren ( Aren van Waarde ) had seen some effects on Adenosine receptors during imaging. Perhaps you could have a chat with him?
Regards,
Nisha
make it more dilute,
make stock in 5% ethanol
further dilute prepare fresh working stock with 0.1% ethanol.