For IPRP of oligos, the TEA concentrations are below 10mM whereas TEAA is used at least 100mM range. What is the possible explanation for lower concentration of one over another?
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
11 July 2024 4,284 0 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
I have made changes in .in1 as well as in .int files but unable to get dos till 20 eV
13 June 2024 9,080 1 View
phenol chloroform extraction method is used for purification of nucleic acids DNA/RNA
03 April 2024 8,268 0 View
phenol chloroform extraction method is used to purify nucleic acids to obtain a good concentration.
03 April 2024 7,244 0 View
as viruses are notorious for being prone to mutation at high rates its very difficult to clone them though general cloning procedures and cells so in order to solve this problem need specific cell...
03 April 2024 9,031 5 View
For the boltaztrap we used the formula for calculating the Seebeck coefficient using a different formula mentioned in doi:10.1016/j.cpc.2006.03.007 and the formula we are using in the experiment...
17 March 2024 2,105 3 View
There are two rooms: Room A and Room B; initially both are dark; contains no light source (of whatsoever). Now, I bought a light source and set it up in the room A, enabling me to see things...
28 February 2024 4,809 10 View
I am performing snake venom fractionation using Reverse-phase HPLC. It generates multiple peaks with different area under the curve. Since I'll be analyzing multiple chromatograms at different...
08 November 2023 9,134 0 View
Dear All, Biotechnologists from The area of Biotechnology. Currently I am serving as HoD in Department of Biotechnology, IMSEC, New Delhi NCR. Is there any person who can help me for two or three...
08 November 2023 2,785 4 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am currently using CASTEP version 2020. When I want to run optical properties calculations with spin-orbit coupling(SOC), I encounter the following error in a pop-up window before the job gets...
02 August 2024 2,890 0 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.
19 July 2024 3,095 0 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
Would an average spot welder suffice for welding tabs for pouch cell electrodes instead of an ultrasonic one? Why would an ultrasonic welder be better than a regular spot welder?
10 July 2024 8,763 0 View
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10 July 2024 3,444 4 View
The diagram suggests that there is a contradiction within the second law of thermodynamics and Carnot's efficiency theory. According to the second law, Carnot efficiency is independent of the...
08 July 2024 7,478 4 View