Hi everyone,
Happy new year :)
I am trying to produce gamma-retroviral vectors with an MSCV-based plasmid and the helper plasmid pCL-Eco via the HEK293T cell line.
Since the produced viruses would be ecotropic, would I be able to concentrate the virus-containing supernatant of 293T cells through high-speed centrifugation?
I am asking the above question due to encountering data regarding the inability of ecotropic viruses to handle high-speed centrifugation due to instability. (https://www.cellbiolabs.com/faq/viral-expression-faq/retroviral-expression-packaging)
Mina Hosseini,
I suppose you are following any particular protocol and if it specifically says not to ultracentrifugation, you may skip that.
You can also try different centrifugation speed and compare that with non-centrifuged ones to observe if that makes any difference.
Hope this helps.
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