What is the lentiviral titer amount and what's the puromycin conc. to use for ARPE-19 transduction?

I am trying to transduce ARPE-19 cell line. I used Lipofectamine 3000 to produce my lentiviral vectors. My 1st attempt at transduction is unsuccessful. I have seeded 0.3 million cell per well in...

17 November 2022 4,430 0 View