We have a compressor 8200. I want to know what kind of airflow (hot/cold) at rear and front of the compressor fan? As one side really hot air is flowing and temperature is not going down in cryocooler.
I am trying for the structure optimization via Gaussian16 DFT calculation, for a system consisting of C,H, N, O and Ce atoms. Where I am using B3LYP/ 6,311G(d,p) C, H, N and O and B3LYP/SDD for...
30 June 2024 9,979 4 View
What basis set/basis sets will be useful for DFT (using Gaussian16) of cerium ion adsorption?
24 June 2024 6,927 3 View
Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...
20 June 2024 9,587 1 View
Kindly mention the protocol
27 May 2024 3,243 0 View
What are the force field parameters for TIP4P-Ew water model? Including the force constant of Oxygen-dummy atom bond length, and hydrogen-oxygen-dummy atom angle? So, basically, what is the...
08 April 2024 3,426 0 View
kindly provide some insights
01 April 2024 4,674 2 View
To culture the U-87-MG cell line, various media formulations are available online. These include: a. Essential Minimum Eagle Medium (EMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL...
07 January 2024 612 0 View
For the past few weeks, I've been attempting to passage U-87-MG cells in our lab. However, after thawing, instead of reaching 80% confluency within 24 hours, they're taking 3-4 days to achieve the...
21 December 2023 509 2 View
I have used Trizol method but there has been huge DNA contamination along with shearing.
17 December 2023 4,745 4 View
I understand that ED-XRF or WD-XRF only allows for high energy photon emission. I am wondering if it is possible to obtain emission in the visible region by attaching an external optical fiber to...
29 November 2023 2,939 3 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View