Hi
I need a control or reference solution for a cardiac troponin T, cTnT, assay. I need it in buffer. It seems cTnT is not stable in solution due to beeing prone to aggregate. This is most likely because of cTnT having a rod-like shape with one net negative end and one net positive end. Full length cTnT is stable in buffers with >6M but this is not possible in this assay. After scouring the net I have come to the conclusion that my best bets are intact troponin-ITC complex or a recombinant cTnT without the last 13 residues. Out of the last residues 6 are positive and none are negative, I suppose this will decrease the aggregation, but it may also effect the structure of the protein. There is no pdb structure for residues 272-298 of cTnT. Does anyone have experience with cTnT controls? Which is to be prefered? Is the recombinant partial cTnT equivalent to full length cTnT? Or are there maybe other tricks to make full length cTnT soluble in buffers? Is there some consensus protocol for cTnT standards?
Any insight into this issue will be utmost appreciated. Thank you in advance.
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