Mean Fluorescent Intensity (MFI) is often used to compare expression of target of interest (TOI) across samples/ cell populations in Flow cytometry. It gives reliable information about expression/ presence of TOI within the experiment. However, more often than not we need to compare samples from different experiments (ex: a sample ran on Flow weeks/months apart). A number of factors could influence MFI of the same TOI in a similarly treated sample such as

1. Tiny differences in staining that are beyond our control (ex: cell viability)

2. Changes in laser power

3. Flow cytometry calibration

4. Differences in compensation

Is there a way we can negate these confounding factors and normalize MFI to make it comparable between experiments. (Something other than normalizing it to a control and reporting as percent control)

Please leave your expert opinions. Thanks!

Shins

More Shinsmon Jose's questions See All
Similar questions and discussions