Mike Watson

4 Questions 10 Answers 0 Followers

Questions related from Mike Watson

Why are negative control cells staining positive in flow assay?

Hi Flow Folks, I've got a puzzle I need your help with. I've developed a flow assay to stain for intracellular neuronal markers. The protocol uses either 2%PFA fix with 0.1% Triton X-100 perm or...

24 June 2019 9,257 1 View

Does anyone titrate their secondary antibodies for intracellular flow?

Hey everyone, I'm working on creating a flow QC panel. I have a few sets of 3 intracellular and 3 surface markers that I know have worked well in the past for ICC and flow applications. My...

28 September 2018 6,442 1 View

My extracted RNA from cells has good A260/A280 purity but poor A260/230 purity. What can I do?

Hello All My Microbio Experts, I'm a total noobie in this field so go easy... I've extracted RNA from cells and the A280/A260 values look good (~2.0 for most samples) which means the RNA is...

01 August 2018 7,085 1 View

Why am i getting a Low RNA yield from cells with RNeasy Plus Mini Kit?

Hey Everyone, I would love some input on an issue I'm facing. I'm doing RNA extraction in advance of RT-qPCR / ddPCR analysis. When I use the QIAGEN RNeasy Plus Mini Kit I'm following the protocol...

26 July 2018 4,383 3 View