How can I calculate packing density of particles by fomulas or softwares?

I have all data of particles, such as size distribution, or numbers. Is there any way that I can calculate the packing density of these particles? Assume that all particles are circular.

05 January 2021 4,272 2 View

How to measure telomerase activities in TISSUE?

Can anyone recommend a protocol to measure the telomerase activity in tissue samples? Before I was able to measure telomerase activity in cell populations using a telomerase activity measurement...

25 November 2020 1,509 1 View

Why did the heat flow of DSC in the 2nd cycle decrease without any decomposition or volatilizing of sample?

My sample was the copper paste contained PEG, and there were no endothermic peaks or exothermal peaks, so I was wondering why did the heat flow in the 2nd and the 3rd cycle decrease? Thanks!

31 August 2020 7,169 3 View

Commercially available TCR-mimic antibody?

18 August 2020 9,995 2 View

Which software is used to plot graphs in engineering books?

Hello guys, I encounter such this plot (attached) in the engineering books, but don't know which software the authors used. It is not only about the plot itself because plotting observed X-Y...

17 July 2020 8,036 4 View

DNase specific to plasmid (circular) DNA?

Is there a specific DNase that digest plasmid (circular) DNA and leaving linear blunt-ended DNA intact? For example after doing CRISPR-Cas9 guided digest of a large BAC vector (~100 kb), I want to...

04 March 2020 4,857 3 View

Which software tool implemented the algorithm for maximum common edge (connected) subgraph?

I need to find the maximum common edge (connected) subgraph of two input graphs. I have implemented them in Python as follow: I construct line graphs of two input graphs then identify the maximum...

16 December 2019 8,088 1 View

Lingo problem, how to use the optimization result of the LP model in the calculation section?

Suppose we have a linear programming model in the below: Model: Max = 3*x1+5*x2 x1

02 October 2019 8,061 3 View

How to mesh a tapered plate in ABAQUS CAE?

Hi all, I am new to ABAQUS CAE and is trying to seek help in RG with regard to my research project. I am trying to mesh a marine stabilizing fin (Basically a tapered plate with hinges-solid) and...

11 April 2018 6,753 6 View

Vapor pressure or Antoine coefficients of LiOH and FeSe?

The experiments I'm doing is gas-phase reaction, so that I wonder to know the vapor pressure of LiOH and FeSe. However, I can't find any data or reference on the internet or the handbook that...

02 November 2017 8,323 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View