good morning all
i have two groups to compare between them : control and persistence bacteria treated with antibiotic (X)... the DNA damage was estimated using comet assay ( Neutral )
what is the estimated from the parameters : olive tail moment , tail DNA % , tail length ??
i supposed that the control because untreated with antibiotic X should contain higher damage than persistence cells !!!!
any ideas
warm regards
Good morning,
If you want to estimate the DNA damage, you need to look at tail DNA %.
For example when I perform the comet assay, I always have:
1) negative control (only cells)
2) positive control (5mM acrylamide in my case)
3) something to adverse the DNA damage, e.g., probiotics (5mM acrylamide + specific probiotic)
In that case, I can estimate the decrease of genotoxicity of acrylamide.
If I don't have probiotic, I can simply estimate the general genototxicity of acrylamide. Try to apply this understanding to your experiment and you should be good :)
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