Hi all,
I would like to infuse a drug (CNO) and monitor neural activity in the same brain region using calcium imaging (fiber photometry) in mice.
Ideally in the in vivo behaving mouse. But I could start with anesthesized mice.
N.B. I can inject CNO, wait 1/2h, and then run fiber photometry.
I have been using cannulas from Plastics One (Invivo1) for local CNO infusions. Typically in the lab my colleagues have been using Doric fiber photometry systems.
Did anybody ever combine the two ? I see Doric has optogenetic/drug infusion dual systems but I couldnt find fiberphotometry/drug infusion dual systems.
Or maybe I should just make it myself...?
Any input or ideas will be highly appreciated !
Hello Marie, why do you need to do a local infusion of CNO? Why don't you inject IP?
Hi Douglas, thanks. Local CNO would be cos I'm trying to target specific projections, not the entire neuron.
Hi Natalia, thanks yes thats a very good point. I think that means I might have to inject the drug long before the recording time (the half-life is long so that might work), thanks for the input. Otherwise yes I also use optogenetics for the same manipulation (I will be doing dual opto/imaging experiments), but unfortunately I also need the DREADD system too.
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