Hello guys,
I infected mice with Salmonella and then I counted the colony numbers from liver and spleen after 6 days. While I was doing this, one of the postdocs mentioned that the distribution of Salmonella might be uneven in the liver. He recommended me use the whole liver instead of a small piece that I cut off.
Is this true? Should I used the whole liver or just a piece?
FYI, I weight those pieces I cut off before homogenize them.
Thank you in advance!
Alexandra Johnson
Yes, definitely. We always homogenize the entire liver when doing bloodstream infections. The liver is huge and has multiple regions, there's no way that bacteria would be evenly distributed through the entire organ.
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