10 Questions 21 Answers 0 Followers
Questions related from Xingchen Dong
Hey guys, I am trying to visualize ASC oligomer ( primarily dimer I believe) during inflammasome activation using western blot technique. But I encountered a problem and failed to see any band...
02 February 2019 3,077 14 View
Hi guys, I tried to transfect poly dA:dT into macrophages to activate Aim2 inflammasome. I setup 4 wells, the first two of which have lps and transfection reagent as negative control. The other 2...
09 September 2018 1,244 6 View
Hi guys, I am trying to see inflammasome activation after delivering flagellin from salmonella into macrophages. I use DOTAP transfection reagent from Roche which is widely used by other...
08 August 2018 2,556 5 View
Hello guys, I infected mice with Salmonella and then I counted the colony numbers from liver and spleen after 6 days. While I was doing this, one of the postdocs mentioned that the distribution...
04 April 2018 8,093 1 View
Hi guys, Where can I find the gene expression level in multiple cell lines, such as I want to see the level of gene A in multiple gastric cancer cell lines? And this level could be either mRNA or...
08 August 2017 9,507 10 View
Hi guys, I am wondering how to know if miRNA is regulated at transcription level? Should i measure pri-miRNA level instead of mature miRNA level?
12 December 2016 5,222 2 View
Hey guys, I recently synthesized an RNA oligo from IDT which has the same sequence as the mature miRNA that i am interested in, but when i transfected the oligo into the cells i didn't see any...
10 October 2016 9,497 10 View
Hey guys, I've been struggling with quantifying miRNA with real-time PCR and the problem now i have is that i couldn't get consistent results. I tried to repeat the experiments literally two days...
10 October 2016 4,471 10 View
Hey guys, Is it necessary to use the small RNA enrichment protocol to do miRNA study in this mirVana miRNA isolation kit? Cuz i found that using the total RNA extraction to perform real-time PCR...
07 July 2016 4,757 6 View
Hi guys, I am trying to quantify the miRNA by using real-time PCR. After the RNA extraction, I need to add the poly A tails to mature miRNAs and I would assume that a poly A tailing kit should be...
07 July 2016 1,891 3 View
