Cold or room temperature PFA for in situ hybridization?

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Luciano Fiore

I always used at RT (but store at 4C and keep out to the light) and works well...

Is better if your PFA is fresh.

Jean-Nicolas Audet

Same here. I heat my PFA to room temperature (or a little higher if my slides are frozen) before using it. I'm guessing 4°C also works fine but the incubation period might have to be prolonged. So my advice would be to follow protocol recommendations of incubation time according to the stated temperature.

Richard Dicasoli

Thanks everyone!