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Questions related from Richard Dicasoli
Hello all, I was wondering if anyone could supply literature pertaining to nerve varicosities in the smooth muscle, particularly in the gut of mice (or the gut of another mammal). I cannot seem...
20 March 2017 9,228 2 View
Whenever I perform an in situ hybridization, black specks appear throughout the tissue within a few hours post mounting with glycerol gel. THey are not present while developing in B4 solution,...
03 November 2016 5,135 3 View
I did an immuno ISH using a DIG-labeled antisense RNA probe and my antibody of interest. The DIG signal was obvious when it came time to wash the slides and add primary antibody to them. I know...
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Is it better to use cold or room temperature 4% paraformaldehyde for in situ hybridization? I have noticed that protocols say it should be cold, but room temperature PFA works well also, so I am...
29 February 2016 3,103 3 View