Richard Dicasoli

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Questions related from Richard Dicasoli

What are the black specks appearing on my in situ hybridization tissue after mounting?

Whenever I perform an in situ hybridization, black specks appear throughout the tissue within a few hours post mounting with glycerol gel. THey are not present while developing in B4 solution,...

03 November 2016 5,064 3 View

Does CFM-1 Plus Mounting medium have a negative effect on DIG-labeled ISH probes?

I did an immuno ISH using a DIG-labeled antisense RNA probe and my antibody of interest. The DIG signal was obvious when it came time to wash the slides and add primary antibody to them. I know...

18 April 2016 2,364 2 View