Richard Dicasoli

4 Questions 8 Answers 0 Followers

Questions related from Richard Dicasoli

Does anyone have literature on smooth muscle nerve varicosities?

Hello all, I was wondering if anyone could supply literature pertaining to nerve varicosities in the smooth muscle, particularly in the gut of mice (or the gut of another mammal). I cannot seem...

20 March 2017 9,230 2 View

What are the black specks appearing on my in situ hybridization tissue after mounting?

Whenever I perform an in situ hybridization, black specks appear throughout the tissue within a few hours post mounting with glycerol gel. THey are not present while developing in B4 solution,...

03 November 2016 5,141 3 View

Does CFM-1 Plus Mounting medium have a negative effect on DIG-labeled ISH probes?

I did an immuno ISH using a DIG-labeled antisense RNA probe and my antibody of interest. The DIG signal was obvious when it came time to wash the slides and add primary antibody to them. I know...

18 April 2016 2,411 2 View

Cold or room temperature PFA for in situ hybridization?

Is it better to use cold or room temperature 4% paraformaldehyde for in situ hybridization? I have noticed that protocols say it should be cold, but room temperature PFA works well also, so I am...

29 February 2016 3,106 3 View