Code for hydrogen from waste water?

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First time secondary research, some points I should keep in mind?

I am conducting secondary research for my dissertation for undergraduation (Psychology) , what should I make sure my proposal consists of? Are there any tips I need to keep in mind?

22 February 2021 6,035 1 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Can anyone provide me with the scoring method and the questionnaire of Work life balance scale by Hayman (2005)?

Greeting! For my MA dissertation, I am thinking of taking Work life balance scale by Hayman. However, I can't find the scoring procedure; also confused about the actual rating taken. If anyone...

23 January 2021 4,074 7 View

Quantitative Research Involves 3 Different Likert Scales, how do I go about data analysis?

This is our first research paper and it is titled "Effect of Peer Connection on Attitude Towards School and Academic Motivation in Higher School Students During Pandemic". We aim to investigate...

30 December 2020 5,450 4 View

My research involves 3 diff scales about peer connect, attitude towards school and academic motivation, how should I go about finding the correlation?

This is the first time I'm conducting research and I'm unsure about the result and scoring process, kindly help

27 December 2020 1,260 3 View

Hi everyone, I am digesting my plasmid with SgrAI enzyme but it showed staractivity everytime?

My plasmid (pJET1.2) contains an insert of around 2969bp. The total size of plasmid 5942 bp. I am cutting it with AhdI, SgrAI and BssHII. Desired bands are of 2969, 1632 and 1432bp. I want to...

15 November 2020 4,761 4 View

Perceived Academic Stress?

Hello, Does anyone have any idea of how this is scored? What would be the average scores? Would would be seen as very low/high? BEDEWY D & GABRIEL A. Examining perceptions of academic...

09 November 2020 2,040 1 View

Does Shannon index represent largest diversity index when the individual belongs to different species and vice versa?

The shannon wiener index and Simpson indices include the measurement of community heterogeneity. Shannon wiener index bapplied information theory. The main measuring object of information theory...

22 October 2020 1,053 7 View

Parameters for magnesium acetate in AMBER MD simulation?

I am trying to use Magnesium acetate [Mg(CH3COO)2] as the salt instead of Sodium chloride [NaCl] in my tleap. Are there any AMBER parameters for acetate ion that I can use?

12 October 2020 931 3 View

Can anyone tell the fast publishing SCI journals?

I want to know the SCI journals of Physics - Computational Physics, Soft Matter Physics, Condensed Matter Physics which provide a fast review process.

12 October 2020 8,911 2 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View